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Complementation tests were performed with various nif mutations for delineating the nif genes. The nif-/nif- heterogenotes were constructed by transferring nif plasmid mutant PRD-nif- from E. coli Jc5466 to various Klebsiella pneumoniae nif- recipients. In addition to those nif genes previously reported elsewhere, a new essential gene for nitrogen fixation,(More)
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