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A simple method for the direct measurement of the catalytic properties of immobilized cells in the flow minicalorimeter, the enzyme thermistor (ET), is presented. A Trigonopsis variabilis strain with cephalosporin C-transforming activity was used as the model system. The yeast cells were immobilized either by crosslinking with a homobifunctional reagent or(More)
Stabilization of immobilizedd-amino-acid oxidase was achieved as follows. YeastTrigonopsis variabilis producingd-amino-acid oxidase was used to deaminate cephalosporin C to glutaryl07-aminocephalosporanic acid. Permeabilized cells were co-immobilized with manganese dioxide by entrapment in (poly)acrylamide gel so that hydrogen peroxide, liberated in the(More)
The possibility of shortening the thermal sterilization time for cultivating media was demonstrated in chlortetracycline fermentation with an industrial strain ofStreptomyces aureofaciens. The medium was artificially contaminated with a mixture of eight strains of G + and G-bacteria isolated from contaminated industrial fermentors, and the following(More)
During fermentation of Acremonium chrysogenum, known cephalosporin-producing organism, there is formed, in addition, the main metabolite cephalosporin C. Its 3-desacetyl derivative, desacetylcephalosporin (DACS). The admixture of this derivative is not desirable. Such factors as the value of pH aeration level and concentration of free glucose influence(More)
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