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The Prp19p-associated complex is essential for the yeast pre-mRNA splicing reaction. The complex consists of at least eight protein components, but is not tightly associated with spliceosomal snRNAs. By a combination of genetic and biochemical methods we previously identified four components of this complex, Ntc25p, Ntc85p, Ntc30p and Ntc20p, all of them(More)
Many applications of gene analysis call for rapid and efficient methods for isolation of genome DNA from whole blood and complex biological samples. Relating to these demands, many protocols have been published (1-6) . However, either the methods involves toxic organic extractions and/or skillful manipulations such as spooling of DNA with an inoculation(More)
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