L. V. Neumyvakin

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Accumulation of free proline (Pro) by bacteria and plants serves as a mechanism protecting these organisms from abiotic stress. We introduced two E. coli genes, proBosm, encoding a mutant variant of the first enzyme of the Pro biosynthetic pathway, gamma-glutamyl kinase, and proA, encoding the second enzyme of the Pro pathway, glutamyl-gamma-semialdehyde(More)
We obtained a spontaneous mutant of Escherichia coli that was characterized by both proline superproduction and the resistance to osmotic stress. The selection of mutants was carried out among 2.5.10(5) clones survived upon plating strain SU1604 containing the sex factor F104, with a chromosome fragment carrying genes proB and proA, on solid modium with the(More)
The idea, design, and implementation of the PR-3 semi-automated dosing apparatus, which is a reliable low-cost mechanism intended for dosed bottling of peptide preparations, are described. This dosing and filling apparatus has proved effective in small-scale production of pharmaceutical preparations. Comparative characteristics of various dosing apparatuses(More)
The design, development, and introduction of an economical and reliable apparatus for dosed batching of syrup formulations of pharmaceutical preparations, i.e., gravitational batching unit URG-1, are described. The apparatus is compact and batches syrups with an accuracy meeting the requirements of All-Union Standard OST 64-992–85. The doser can be used in(More)
Effects of recombinant bacteria Rhizobium fredii/pNSA, content of proBosm proA operon, and R.fredii/pLVA, content of proBA wild type operon, on soybean plants were studied. R.fredii BD32/pNSA were found to synthesize more proline and to express higher osmotolerance than R.fredii BD32/pLVA or wild type bacteria. Plants infected with R.fredii Bd32/pNSA were(More)
Medicinals not sterilized in the course of their production can be contaminated by microorganisms. The level of microbial contamination of nonsterile medicinal preparations (NSMPs) provides one of the most important criteria for the quality control of pharmaceuticals. Various methods are used in order to ensure proper microbiological purity of NSMPs. In(More)
The proA proline biosynthesis gene of thermophilic bacterium Thermus ruber was cloned and sequenced, and several properties of the encoded enzyme, gamma-glutamylphosphate reductase (GPR) were studied. The proA open reading frame (ORF) was of 1286 bp. Nucleotide sequence analysis revealed the ATG initiation codon in position 36 and the TTA termination codon(More)
A model of venous thrombosis on the rabbit marginal ear vein was developed. It is based on occlusion of a segment of the left or right ear vein followed by injection of thrombin into this segment. Thrombolytic activity of longolytin applied externally onto the thrombotic venous segment was evaluated. The drug 2-fold shortened the time of clot lysis and(More)
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