L V Miriasova

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The fractional composition of the vaccine preparation obtained by the hydroxylamine treatment of K. pneumoniae 204 cells was studied with the use of gel chromatography in sepharose CL-6B. The preparation was shown to form 2 peaks, only the first high molecular peak being serologically active. The method for the treatment of the culture, obtained directly(More)
Whole-cell preparations, obtained from the microbial mass of K. pneumoniae grown by batch and continuous cultivation at a dilution rate of 0.24 and 0.41 hr-1 and used in animal experiments for oral administration, were shown to have different serological activity. The preparation obtained from biomass grown by continuous cultivation at a dilution rate of(More)
K. pneumoniae lipopolysaccharide (LPS) was isolated from biomass grown under the conditions of controlled multicycle and continuous cultivation. A considerable yield of LPS containing the minimal amount of protein and nucleic acid admixtures was obtained from biomass grown by continuous cultivation with the concentration of glucose in the medium equal to 20(More)
The drainage-filling cultivation process for three Shigella flexneri vaccine strains in small-capacity fermenters has been developed. The study has shown that after hydroxylamine treatment the yield of the antigenic component, calculated per 1000 million microbial cells, is tenfold higher in comparison with the traditional method.
Silicon, dysprosium, germanium, yttrium, iron, cobalt, samarium, lutecium oxides, as well as the mixture of 8 metal oxides, at a concentration of 20 g/l were found to produce a stimulating or inhibiting effect on the growth of K. pneumoniae strains 204 and K-9. Silicon, dysprosium, germanium and yttrium oxides were shown to stimulate the growth of K.(More)
The authors studied the antigenic activity of Bordetella pertussis during stab cultivation. For the first time there were revealed changes (oscillations) of antigenic activity: formation of 3 peaks of which the middle was the highest. A possibility of improving the quality of pertussis vaccine by using biomass at the height of the peak was experimentally(More)
The study of the ultrastructure of meningococci (strain C0638) in the process of submerged batch cultivation permitted the authors to follow changes in the fine structure of these microorganisms in different phases of the growth of the culture. Thus, the meningococcal populations retained their ultrastructure most intact during the exponential phase and the(More)
The article deals with the study of the kinetics of the processes occurring in the population of B. pertussis grown in a semisynthetic culture medium. On the basis of the mathematical description of the specific growth rate the factors permitting the regulation of the processes of the cultivation of B. pertussis have been revealed. New prolonged multicyclic(More)
In the technology of the cultivation of K. pneumoniae vaccine strain 204 with a view to obtaining biomass for the production of antigenic preparations the traditionally used culture medium with full nutritional value has been replaced by the alternative variant of synthetic medium. The specific physiological and morphological features of this strain grown(More)