L Turpeenoja

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The age-dependent modifications of synaptosomal plasma membrane protein composition in three different rat brain regions (cerebral cortex, cerebellum and striatum) at various ages (4, 12 and 24 months) were studied. The proteins were separated by gel-electrophoresis and the quantity of the different polypeptides was determined densitometrically from the(More)
The effect of epidermal growth factor (EGF) and insulin on DNA, RNA, and cytoskeletal protein labeling in primary rat astroglial cell cultures was investigated. Cultures were grown for 15-30 days in vitro in 10% fetal calf serum (FCS)-supplemented medium and then maintained in serum-free basal medium (DMEM) supplemented with fatty acid-free bovine serum(More)
This study investigated the effects of epidermal growth factor (EGF) on the labeling of various RNA species and of nuclear proteins in primary rat astroglial cell cultures. After 12 hours of EGF treatment in serum-free medium or chemically defined medium, significant increase in RNA labeling, and also in acid-soluble radioactivity and RNA content, was(More)
Calf brain synaptic plasma membranes (SPM) were saturated under extracellular conditions with [14C] glutamic acid, [14C] GABA, [14C] taurine, [3H] tyrosine or [14C] norleucine and the resulting labelled membrane complexes fractionated by a differential extraction procedure using 0.9% NaCl, distilled water, n-butanol-water, 0.05 M NaOH and 0.5% Triton X-100(More)
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