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A library of cloned fragments representing nearly the entire frog virus 3 (FV 3) genome (99.65%) has been constituted. Individual plasmid recombinants, labeled by nick-translation, were hybridized to Southern blots of genomic FV 3 DNA fragments obtained with XbaI, HindIII, SmaI, and SalI. From these results physical maps were generated and the distribution(More)
Frog virus 3 (FV3) of the Iridoviridae family has the most highly methylated DNA of any DNA virus. Its transcription presents interesting features. Although the host RNA polymerase is required for immediate early (IE) transcription, the viral DNA is not infectious and a protein component of the virion is necessary for the transcription of at least some IE(More)
The detailed organization of the RNAs transcribed from a region of the FV 3 genome (Sa/I-F fragment and adjacent sequences) has been determined. The information was derived from the cell-free translation of hybrid-selected RNA to locate the genes encoding specific polypeptides, RNA filter hybridization to size the transcripts, and S1 nuclease mapping to(More)
A modified procedure for the transfer of electrophoretically-separated proteins from sodium dodecyl sulfate (SDS)-polyacrylamide gels onto nitrocellulose filters has been developed. During the diffusion mediated transfer, the SDS-protein complexes were maintained and SDS was added to the buffer. This increases the number of polypeptide species bound to the(More)
In a productive infection, frog virus 3 (FV 3) DNA was synthesized in both the cell nucleus and cytoplasm. The infection was aborted in arginine-starved Chinese hamster ovary cells and viral DNA replication was then restricted to the nuclear compartment. It has been demonstrated that the newly synthesized FV 3 DNA present in the nucleus is of genomic size.(More)
The gene encoding the major capsid polypeptide (MCP 48) of frog virus 3 (FV 3) has been mapped on the viral DNA. Late FV 3 messenger RNA, hybrid-selected by the SalI-F fragment or a subset of these sequences, BamHI-L and -W fragments, directed the synthesis in vitro of a 48 000 mol. wt. (48K) polypeptide. This product was recognized by monospecific(More)
The influence of temperature on the transcription of the frog virus 3 genome was studied in CHO cells infected both at 29 and at 37 degrees, the nonpermissive temperature for virus multiplication. It was definitely established that late genes were not transcribed at 37 degrees. Although immediate early genes were expressed at 37 degrees, their transcription(More)
Multiplication of frog virus 3 (FV 3) occurs in mammalian cells provided they are incubated at temperatures lower than 33 degrees. The expression of the viral genome at supraoptimal temperatures was followed by analyzing the polypeptides produced in CHO-infected cells and comparing with those obtained under restrictive conditions provoked by amino acid(More)
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