L. T. Lubomirov

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1. Urocortin is an endogenous vasodilator although the mechanism of vasorelaxation is not completely understood. The hypothesis that an alteration of smooth muscle calcium concentration is involved was tested using isometric tension recording and calcium fluorimetry. The relationship between contraction and intracellular calcium was also estimated. 2.(More)
Urocortin, a peptide hormone related to the corticotropin releasing factor, is suggested to be involved in blood pressure regulation by dilating the peripheral blood vessels. In rat tail arteries, urocortin-induced vasodilation is due to a decrease in myofilament Ca2+ sensitivity the mechanism of which is still unclear. In this study, the hypothesis was(More)
AIM Our aim was to study the new signalling pathway of ghrelin in the guinea-pig femoral artery using the outward I(K) as a sensor. METHODS Whole-cell patch-clamp experiments were performed on single smooth muscle cells, freshly isolated from the guinea-pig femoral artery. The contractile force of isometric preparations of the same artery was measured(More)
In contraction studies corticotropin-releasing hormone (CRH) was found to relax ileal but not gastric and jejunal smooth muscles of the guinea-pig, precontracted with BaCl2. Under whole-cell patch-clamp conditions, CRH concentration-dependently activated Ca2+-sensitive K+ currents (I K) with ED50=20 pM at 100 nM and ED50=0.13 pM at 500 nM intracellular Ca2+(More)
Thyrotropin-releasing hormone (TRH) is an amidated tripeptide, which is secreted by the hypothalamus and stimulates the release of thyrotropin and other tropic hormones from the anterior pituitary [1]. TRH-like immunoreactivity is detected not only in the central nervous system but also in the body fluids of mammals, including humans [2], as well as in(More)
Urocortin, a novel vasodilatory peptide related to the corticotropin-releasing factor (CRF) increased cAMP levels to 220.8 ± 27.6% of control in rat tail arteries. The effect was completely abolished by the adenylyl cyclase inhibitor, SQ22536 (100 μM). Urocortin also decreased phosphorylation of the regulatory light chains of myosin (MLC20) in rat tail(More)
Thyrotropin-releasing hormone (TRH) is released in high concentrations into gastric juice, but its direct effect on gastric smooth muscles has not been studied yet. We undertook studies on TRH effect on gastric smooth muscle using contraction and patch clamp methods. TRH was found to inhibit both acetylcholine- and BaCl2-induced contractions of gastric(More)
This study aims to reveal the reason for the increased force of 5-hydroxytryptamine-induced contraction of endothelium-denuded skeletal muscle arteries of diabetic rats in the presence of perivascular adipose tissue (PVAT). Our data on rat gracilis arteries show that i) PVAT of skeletal muscle arteries of healthy and diabetic rats releases hydrogen peroxide(More)
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