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Simian adenovirus SA7 heated for 30 minutes at 70° C retained part of its ability to induce tumors in newborn hamsters. Tumors were also induced by DNA extracted from the heated virus. However, neither residual replicating, i.e. cytopathogenic, nor transforming and T-antigen-inducing activities could be detectedin vitro in CV-1 and hamster embryo cells(More)
Following apparently effective inactivation of herpes simplex virus types 1 and 2 at 56 or 70 degrees, significant infectivity was found upon viral transfection using the calcium-dimethylsulfoxide technique. Infectivity was quantitated in RSB-1 (rabbit skin fibroblast) and BHK-21 cells, and dose-response curves were found to depend on both the cell system(More)
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