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These studies were performed to determine when, during fetal or postnatal life, rat pancreatic acinar cells acquire their capacity to respond to cholinergic drugs. The release of amylase, lipase, and chymotrypsin from rat pancreas in response to urecholine was measured in vitro. Fetal and newborn pancreas did not respond to 10(-5) M urecholine; 3-day- to(More)
Ultrastructural morphometric techniques were used to quantify pericyte degeneration in retinal and uveal capillaries of streptozotocin-diabetic rats in order to assess the suitability of this small rodent model of diabetes for studies of the pathogenesis of microvascular eye disease in diabetic humans. Male, Sprague-Dawley rats were killed by intraaortic(More)
This study was undertaken to establish if there is a correlation between the ontogenesis of cholinesterase activities and the cholinergic muscarinic receptors in the development of rat pancreas. Two different cholinesterase activities were detected in the pancreas: a true acetylcholinesterase and a pseudocholinesterase. The ratio of acetylcholinesterase to(More)
Biopsy specimens from the myocardium were examined in a series of 145 patients who had elected coronary arterial bypass grafting. The patients were divided into three groups; 1) overtly diabetic (OD) patients; 2) chemically diabetic (CD) patients, who demonstrated impaired glucose tolerance only when stressed with a sugar load; and 3) normoglycemic,(More)
In this study, dispersed rat pancreatic acini exhibited secretin subsensitivity in their capacity to release amylase after preexposure to increasing concentrations of the muscarinic cholinergic agonist carbamylcholine. The present study also explores the potential mechanisms involved in this cellular desensitization phenomenon. Secretin subsensitivity of(More)
This study examines the effects of cerulein-induced acute pancreatitis on the secretory response of rat pancreatic acini to carbamylcholine and concentration of acinar muscarinic receptors. Rats were injected subcutaneously every 8 hr with cerulein, 12 μg/kg, for two days. They were sacrificed 2 and 4 hr after the first injection, 4 hr after the second and(More)
K(+) channel blockers such as 4-aminopyridine (4-AP) can be toxic to neurons; the cellular mechanism underlying the toxicity, however, is obscure. In cultured mouse cortical neurons, we tested the hypothesis that the toxic effect of 4-AP might result from inhibiting the Na(+),K(+)-ATPase (Na(+),K(+)-pump) and thereafter induction of a hybrid death of(More)
Pancreatic response to urecholine was studied in rats which were fed maternal milk only until they reached 27 days of age. A group of pups which remained all the time with their mother were weaned at 21 days; over that nursing period, their pancreatic amylase concentration increased gradually, lipase started to decrease from day 23 while chymotrypsin(More)
Alzet mini-pumps were used to study modulation of rat pancreatic muscarinic receptors and the acinic secretory response to long-term N-methylscopolamine (NMS) treatment. Infused intraperitoneally (i.p.), NMS, 25 mg.kg-1.day-1 for 14 days, resulted in a shift to the left in the amylase dose-response curve to carbamylcholine (Cch) and an increase in receptor(More)
Regional 125I-albumin permeation and glomerular structural changes were assessed in male Sprague-Dawley rats with diabetes and/or hypertension. All rats underwent unilateral nephrectomy 2 weeks after induction of diabetes with streptozotocin. At the same time, one-half of the nondiabetic and diabetic animals were placed on 1% saline drinking water and given(More)