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The use of avidin-biotin interaction in immunoenzymatic techniques provides a simple and sensitive method to localize antigens in formalin-fixed tissues. Among the several staining procedures available, the ABC method, which involves an application of biotin-labeled secondary antibody followed by the addition of avidin-biotin-peroxidase complex, gives a(More)
A highly sensitive immunoenzymatic technic is presented. The method involves three sequential steps: (1) primary antibody, (2) biotin-labeled secondary antibody, and (3) avidin-biotin-peroxidase complex. Avidin, an egg white protein, has four binding sites for the low-molecular-weight vitamin biotin. Many moieties of biotin can be coupled to the peroxidase(More)
Avidin has an extraordinary affinity for the small-molecule vitamin biotin. Covalently coupling biotin or avidin to peroxidase molecules does not interfere with their normal biochemical functions. The avidin or biotin molecules, either peroxidase conjugated or unconjugated, can be brought to the antigen sites by means of an antiavidin antibody. Several(More)
Conjugated and unlabeled peroxidase antibody methods have proven to be quite satisfactory in localizing sites of antigen-antibody reaction. The use of avidin-biotin-peroxidase complex (ABC), as well as protein A, can contribute significantly to the field of immunohistochemistry. The sensitivity and specificity of several immunohistochemical methods is(More)
Carbohydrate residues in tissue can be localized by a very sensitive, specific, and simple technique, utilizing a biotin-labeled lectin followed by an avidin-biotin-peroxidase complex (ABC). Distribution of peanut receptors in benign and malignant tissues was found to be quite different. Normal colonic mucosa occasionally shows small dot staining in the(More)
Immunoperoxidase staining for IgA, IgG, IgM, and secretory component (SC) was performed on ten cases each of medullary carcinoma and infiltrating duct carcinoma of breast. Plasma cell-rich stroma of medullary carcinoma was found to contain predominantly IgA plasma cells. Tumor cells also contained IgA and SC. In contrast, the few plasma cells of(More)
The staining reaction of a panel of lectins in paraffin embedded lymph node specimens of diffuse large cell lymphoma was studied in relation to survival. In 47 of 49 patients, varying degrees of lectin binding were observed with Ricinus communis agglutinin (RCA), crude extract of Arachis hypogaea (c-PNA), Concanavalin ensiformis A (Con A), Triticum vulgaris(More)
Bessey, 0. A. & Lowry, 0. H. (1946). J. biol. Chem. 163, 633. Boell, E. J. & Schen, S. C. (1954). Exp. Cell Re8. 7, 147. Bruel, D., Holter, H., Linderstrom-Lang, K. & Rozits, K. (1946). C.R. Lab. Carleberg (Sir. chirm.), 25, 289. Conway, E. J. (1947). Micro-Diffu8ion and Volumetric. Error, 2nd ed. London: Crosby Lockwood. Doyle, W. L. & Omoto, J. H. (1950).(More)
sucrose solutions seems to agree with some results of Greenfield & Price (1956), who stated that during the preparations ofsamples ofmitochondrial catalase some enzyme was 'eluted' from the particles by the sucrose solutions used. During the incubation of granule suspensions in sucrose no diminution in the number of granules presen-t could be seen, and(More)