L R Kreisman

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The higher-order assembly of the approximately 30 nm chromatin fibers into the characteristic morphology of HeLa mitotic chromosomes was investigated by electron microscopy. Transmission electron microscopy (TEM) of serial sections was applied to view the distribution of the DNA-histone-nonhistone fibers through the chromatid arms. Scanning electron(More)
The relationship between the surface protuberances of mitotic chromosomes isolated from HeLa cells and the underlying fiber organization was investigated by scanning electron microscopy (SEM). Chromosomes were isolated in the presence of 5.0 mM Mg2+ by a method which avoids the use of organic solvents and extremes of pH. Chromosomes in 5.0 mM Mg2+ are(More)
Scanning electron microscopy (SEM), as well as transmission electron microscopy (TEM), has been utilized to determine how the surface structure of mitotic chromosomes is related to the organization of the 30 nm chromosomal fibers. SEM revealed the surfaces of isolated, HeLa cell chromosomes to possess a knobby substructure with chromosomes prepared for EM(More)
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