L. N. Sinyashina

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Bordetella phages give rise to changes in the important phenotypic properties of all three Bordetella species. The changes depend on the phage system used. The treatment of Bordetella parapertussis with phages from B. pertussis led to toxic clones with manifold combinations of agglutinogens specific for all three Bordetella species. Under the influence of(More)
The role of yadA, invA, and psaA genes in virulence was studied using a Yersinia pseudotuberculosis strain isolated from a patient with Far-East scarlatinoid fever as a model. Isogenic single, double, and multiple mutants of the studied genes were constructed, in which wild-type alleles were inactivated by the insertion of various antibiotic-resistance(More)
Avirulent B. pertussis bacteria containing IS elements in the bvgAS operon were detected during the study of whooping cough patients and bacilli carriers. The present work is devoted to the study of the accumulation dynamics and the mechanisms of generation of persistent forms of the B. pertussis bacteria in lower monkeys as the most adequate model for(More)
Recombinant (attenuated) bacteria Bordetella pertussis that contains a knock-out mutation in dnt gene and produces a nontoxic derivative of pertussis toxin have been constructed. Immunological properties of mutant bacteria B. pertussis of KS strain have studied. It is demonstrated that recombinant bacteria B. pertussis of KS strain devoid of activity of(More)
Several plasmids that contain genetically marked variants of the TnBP transposon isolated from Bordetella pertussis have been constructed based on a thermosensitive plasmid. The frequencies of the integration of these plasmids into an E. coli K12 chromosome at a nonpermissive temperature (42°C) have been studied. A decline in the frequency of RSBP-induced(More)
Significant growth in the incidence of laboratory proven pertussis in adolescents and adults worldwide, the detection of asymptomatic carriage and intracellular survival of Bordetella pertussis in vivo, and the dependence of this process on the virulence of the causative agent denote the possibility of the persistence of bacteria that belong to the genus(More)
The duration and dynamics of accumulation of insertional Bordetella pertussis Bvg− mutants were studied in the lungs of laboratory mice after intranasal and intravenous challenge by virulent bacteria of the causative agent of whooping cough. The ability of the virulent B. pertussis bacteria to remain for a long time in the body of mice was established.(More)
A new bacteriophage ϕK of microorganisms belonging to the genus Bordetella was isolated from cells of the earlier characterized strains 662-2 (1 and 2) obtained upon phage conversion of B. parapertussis 17 903 cells by B. pertussis bacteriophage ϕ134. Bacteriophage ϕK is identical to previously described Bordetella bacteriophages ϕT, ϕ134, and ϕ214 in(More)
A method of monitoring the sequential events of IS481 transposition into the ctag site of bvg operon of Bordetella pertussis has been developed. Reproduction of virulent B. pertussis cells in vitro is accompanied by intrachromosomal site-specific IS481 transposition, which, in turn, results in inactivation of bvg operon of the causative agent and cell(More)
A growth of pertussis morbidity is observed in many countries of the world against the background of mass vaccindtion. Forms of the disease course have changed. Atypical forms of pertussis occur predominately in adolescents and adults. Asymptomatic carriage of the causative agent has been established. Infection of infants with. BordetelIa pertussis bacteria(More)