L N Reshetnikova

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A scheme for isolation of highly purified type F Cl. botulinum toxin has been developed. The scheme allows one to obtain the toxin with specific activity of 1.5--4.0 X 10(7) Dlm/mg protein, molecular weight of 150,000 daltons, a typical protein UV spectrum and the optical density ratio E280/E260 = 1.98.