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OBJECTIVES This prospective study focused on the evaluation of antiparasitic therapy in chronic Chagas' disease, monitored by PCR. PATIENTS AND METHODS One hundred and eighty-one patients, all seropositive for Trypanosoma cruzi infection, underwent specific chemotherapy with benznidazole. Twelve patients were classified as young (2-19 years), 122 patients(More)
Telomere length and telomerase activity are important factors in the pathobiology of human diseases. Age-related diseases and premature aging syndromes are characterized by short telomeres, which can compromise cell viability, whereas tumour cells can prevent telomere loss by aberrantly upregulating telomerase. The zebrafish (Danio rerio) offers multiple(More)
Leishmania spp. infection was investigated in tissue samples of wild carnivores from the Spanish Basque Country (BC), by PCR and DNA sequencing. The region is at the northern periphery of Leishmania infantum endemic Iberian Peninsula and infection in the dog (reservoir) or other species has not been previously reported. Leishmania kinetoplast DNA was(More)
Chagas disease is a disease endemic in Latin America, caused by the parasite Trypanosoma cruzi. Benznidazole is the most commonly used drug for the etiological treatment of the disease although its effectiveness varies according to the phase of the same and toxic side effects are frequent. This prospective study describes the side effects of benznidazole(More)
An epidemiological study was carried out to investigate asymptomatic Leishmania infantum infection by PCR and ELISA in wild rabbits, humans and domestic dogs in southeastern Spain. Seroprevalence was 0% (0/36) in rabbits, 2% (13/657) in humans and 7% (14/208) in dogs. The prevalence of PCR-positives was 0.6% (1/162) in rabbits tested in a wide range of(More)
BACKGROUND In this longitudinal cohort study we evaluated the congenital transmission of Chagas disease (CD) in a nonendemic area. The aim of this work was to analyze the predictive value of a Trypanosoma cruzi-positive polymerase chain reaction (PCR) result in pregnant women for the diagnosis of vertical transmission and to evaluate the use of PCR as a(More)
1 Rev Esp Quimioter 2012;25 (1):1-3 post-treatment, which was deemed to reflect the effectiveness of the treatment to clear parasites better than serology. Moreover, PCR allowed treatment failure to be ratified – in our case in 6.9% of the patients, who shifted to positive at the end of the follow-up period. Although PCR is a sensitive and specific tool for(More)
The innate immune response from diamniotic and dichorionic twin brothers congenitally infected with Trypanosoma. cruzi (strain DTU-V) who displayed different clinical symptomatology was studied. While Brother I manifested severe cardiac and digestive disorders, the Brother II showed slight splenomegaly. The secretion level of IL-1β, TNF-α, IL-12, IL-10,(More)
86 Rev Esp Quimioter 2012;25 (1):74-75 Nifurtimox, is a nitrofuran compound with a trypanocidal effect against both trypomastigote and amastigote forms of T. cruzi. Its antiparasitic activity is due to the formation of toxic derivatives of oxygen when nifurtimox is reduced. T. cruzi presents low levels of reduced glutathione and lacks catalase and(More)