L Labieniec-Pintel

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The right-hand 80% of the genome of minute virus of mice (MVM) was cloned into the bovine papillomavirus type I shuttle vector and used to transfect mouse C127 cells. Transformed lines were isolated that efficiently produce both authentic MVM capsid proteins at a ratio similar to that seen in a normal viral infection, and these proteins assemble into intact(More)
In-phase single-codon insertion mutations were constructed in the open reading frames of the NS-1 and NS-2 genes of the autonomous parvovirus minute virus of mice. A viral mutant containing an isoleucine insertion exclusively within NS-1 between residues 229 and 230 was isolated that produced approximately 3 orders of magnitude fewer plaques at 39 degrees C(More)
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