L. L. Litinskaya

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Measurements were made of the pH of cytoplasm and lysosomes of cultured skin fibroblasts from healthy donors and from patients with lysosomal storage diseases (mannosidosis, Fabry's disease, and Krabbe's disease), and the effects of sucrose loading on normal fibroblasts were studied. The cytoplasmic pH of the pathological cells did not differ from control(More)
9. 10. I. N. Marokko, M. T. Khlopushina, E. M. Lysenkova, et al., Byull. l~ksp. Biol. Med., 98, No. 12, 713 (1984). T. Yu. Mizina, G. A. Dokshina, and L. N. Karlova, Radiobiologiya, 26, No. 5, 708 (1986). A. G. Platonov, M. Ya. Akhalaya, and L. I. Deev, Radiobiologiya, 19, No. 5, 649 (1979). No E. Polyakova, O. A. Gromova, and I. B. Tsyrlov, Vopr. Med.(More)
Scanning integrating visible cytospectrophotometry of gallocyanin-, amido black-, fast green- or ammoniacal silver-stained sections of various areas of the central nervous system of adult rats sacrificed at 4 h intervals has demonstrated the presence of circadian changes in RNA, total protein and total histone content as well as in arginine-rich to(More)
Many lysosomal storage diseases have been shown to be based on a hereditary deficiency of one or other lysosomal hydrolase, or factors essential for the functioning of enzymes of this group. For example, the most numerous group of lysosomal storage diseases, the glycosidoses, is characterized by a wide range of defects involving glycosidases: reduced(More)
We compared the Feulgen hydrolysis curves (37 degrees C, 5 M HCl) of human blood lymphocytes fixed by the following four methods: 96% ethanol, 60 min at 20 degrees C; ethanol-acetone, 1:1, 120 min at 4 degrees C; ethanol-glacial acetic acid mixture (3:1), containing 2% of formaldehyde (EAF), 90 min at 20 degrees C; and ethanol-glacial acetic acid (3:1), 60(More)
Although the Feulgen reaction has been in use for a long time its mechanisms are still largely unexplained [3]. Investigations conducted in the 1970s shed light on some aspects of the kinetics of liberation of aldehyde groups binding Schiff's reagent during acid hydrolysis of DNA [7, i0]. On this basis was formulated the concepts of "chain with stable(More)
The effect of hyperthermia on pH of cells cultivated at varying pH of the medium and under different conditions of oxygenation has been studied. Chinese hamster fibroblasts and pig embryo kidney cells were used. The intracellular pH (pHi) was determined by the microfluorimetric method using fluorescein diacetate. The required temperature (37 degrees C and(More)
Changes in the size and dry mass of glioma C-6 intact cells in culture were investigated for 35-50 min at 5 min intervals by means of vital cytointerferometry. Rhythmic variations in the size, dry mass and protein concentration were thereby revealed in glioma cells. These variations fall into the category of circahoralian ones. While considerable variations(More)
Cell populations of 19 human brain tumors were studied by the combination of autoradiographic and cytophotometric methods. In some tumors there were up to 20% polyploid cells, but in the majority of cases the number of labeled polyploid cells was minimal. In 17 cases, labeled cells were within the range 2c to 4c or 1c to 3c: i.e., mainly near-diploid or(More)
We compared the Feulgen hydrolysis curves (37° C, 5 M HCl) of human blood lymphocytes fixed by the following four methods: 96% ethanol, 60 min at 20° C; ethanol-acetone, 1∶1, 120 min at 4° C; ethanolglacial acetic acid mixture (3∶1), containing 2% of formaldehyde (EAF), 90 min at 20° C; and ethanol-glacial acetic acid (3∶1), 60 min followed by 5% chrome(More)
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