L. G. Zhdanova

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A simple method was developed for end-point fluorescence detection of the 735G → A mutation of the 5′-splice donor site of intron 14 of the dihidropyrimidine dehydrogenase gene (DPYD). The method was based on allele-specific PCR with duplex Scorpion primers. The genotyping results obtained by the fluorescent end-point PCR technique completely coincided with(More)
1 The gene encoding the S100b protein is expressed solely in glial cells (astrocytes and Schwann cells) of the central nervous system [1]. Its protein product, with respect to molecular weight, is the major protein of the S100 superfamily in the brain [2]. It is believed that the S100b protein (in the form of disulfide homodimer) induces branching and(More)
The structural organization of murine S100b gene was published in [1]. Later it was demonstrated that there exists a correlation between the expression of Sl00b encoding a brain-specific protein and some complex behavioral reactions of organisms [2-4] as well as some neurological anomalies such as the Dawn syndrome [5] and the Alzheimer disease [6]. The(More)
We describe an approach to the synthesis of TaqMan oligonucleotide probes that is based on Cu(I)-catalyzed alkyne-azide cycloaddition (CuAAC) click chemistry when oligonucleotides containing an internal alkynyl group at the pyrimidine position are labeled post-synthetically with a f luorescent azide. TaqMan probes were constructed with f luorescein in(More)
It was shown that in continuous cultivation of typhoid bacilli, by changing the rate of dilution and the regimens of carbohydrate and oxygen supply it was possible to determine conditions under which the most highly immunogenic vaccines were obtained and the maximal synthesis of antigens was observed. The regimen optimal for obtaining the highly immunogenic(More)