L G Venkov

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Although the Bradford protein estimation assay has found wide distribution, it has a number of drawbacks, which in some cases have been shown to produce erroneous results upon comparison to other, more precise, methods for protein estimation. It was found that the underestimation of the protein content of membrane-containing fractions cannot be overcome by(More)
The LDH isoenzymes of cell organelles (nuclei, mitochondria, microsomes, cytosol) obtained from neuronal- and glial-enriched fractions were determined by disc polyacrylamide electrophoresis and stained for enzyme activity. The quantitative estimation was made either by elution of isoenzymes or by densitometric measurements. The neuronal fraction was found(More)
During synaptogenesis the plasma membrane of neurons undergoes considerable changes and large portions of it develop to synaptic membranes. This transformation is brought about by biochemical and morphological changes. The aim of the present investigation was to study by morphological methods the in vivo changes of some basic components of the neuronal(More)
The damage and repair of rat brain DNA was studied in vivo after a single carcinogenic dose of ethylnitrosourea. Fragmentation of the brain DNA produced by this carcinogen was demonstrated on alkaline sucrose gradients. By the 24th hrs after treatment with ethylnitrosourea the single-strand damage to DNA was not completely repaired. As the highly(More)
Growth cones were isolated from the forebrains of 1, 5 and 9 days-old rats. The ultrastructural characterization of the obtained subcellular fractions reveals that two of them (GC1 and GC2) contain predominantly growth cones. It was found that the protein content of the membranes contained in these fractions increases 7.5 times, while in whole forebrain the(More)
l'lic tiilf~~sion and conver\ion of ACli fi-on1 one form Into anotlier responsible ior tlic t~.;uism~ssion oi licrvc ~ r n p ~ ~ l \ c \ in the cholincrgic iynxpsc's is describeti b> mcam offi\c \iiii~iltancous ortiinor? d~lkrcnt~al ctluatlon\.INTRODLJCTION The I;)llowi~ig basic liqpotlieses with respect t o tlie 11-:lnsrnission of ncrcc impulses in the(More)
Membrane and soluble protein fractions were obtained from forebrain, midbrain and hindbrain of embryos or neonatal rats. The amount of the protein of the corresponding brain parts was followed up as a function of DNA content. Age-related changes of the concentration of over 50 protein bands were observed in all three brain parts. There are also bands which(More)
The molar concentrations of the catalytic sites of serum cholinesterase (ChE--EC 3.1.1.8.) and cholinesterases (ChEs-acetylcholinesterase (AChE)--EC 3.1.1.7. and ChE) from brain and perfused liver of male birds, rats, swine and sheep were determined. A positive correlation between the molar concentrations of the catalytic sites of ChEs and the resistance of(More)