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Serum and mucosal antibody responses and protection in pigs vaccinated against Mycoplasma hyopneumoniae with vaccines containing a denatured membrane antigen pool and adjuvant.
OBJECTIVE To investigate the protective efficacy of a pool of denatured membrane protein antigens of Mycoplasma hyopneumoniae (J strain) in the molecular size range 70 to 85 kDa (F3 antigen) inExpand
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Serum and mucosal antibody responses against Mycoplasma hyopneumoniae following intraperitoneal vaccination and challenge of pigs with M hyopneumoniae.
Pigs were immunised intraperitoneally when six weeks old and again at about 10 weeks old with killed Mycoplasma hyopneumoniae antigen prepared in an oil adjuvant. The pigs were challenged with live MExpand
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Serum antibody response to Mycoplasma hyopneumoniae measured by enzyme-linked immunosorbent assay after experimental and natural infection of pigs.
Studies were conducted under experimental and field conditions to determine the effect of infection with M. hyopneumoniae on the immune response in serum as measured by ELISA. Following intratrachealExpand
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An improved enzyme linked immunosorbent assay (ELISA) for the detection of porcine serum antibodies against Mycoplasma hyopneumoniae.
An ELISA for the detection of serum antibodies to Mycoplasma hypopneumoniae in pigs and based on a 43 kDa purified protein derived from the cytoplasmic membrane of M. hyopneumoniae strain J isExpand
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PH Control in the fluorometric assay for selenium with 2,3-diaminonaphthalene.
Inconsistencies in reproducibility and duplication in the fluorometric assay of selenium in biological materials with 2,3-diaminonaphthalene (DAN) were investigated. These were attributed toExpand
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Evaluation of an enzyme-linked immunosorbent assay for the detection of Mycoplasma hyopneumoniae antibody in porcine serum.
An enzyme-linked immunosorbent assay (ELISA) for detecting antibody to Mycoplasma hyopneumoniae in porcine serum is described. The results are presented as an ELISA ratio, calculated by dividing theExpand
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Intraperitoneal vaccination of pigs to control Mycoplasma hyopneumoniae.
In a field trial at a commercial piggery 22 pigs were vaccinated intraperitoneally, at 30 days and 60 days old, with formalin-killed Mycoplasma hyopneumoniae plus adjuvant. Two other groups of theExpand
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Origin of antibody in porcine bile after intraperitoneal immunisation.
Pigs were immunised intraperitoneally with ovalbumin (OVA) in Freund's complete adjuvant and killed between 52 and 71 days later. Sera, bile and spleen and liver tissue were collected at slaughter.Expand
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Specific antibody containing cells in the porcine respiratory tract following intraperitoneal and intratracheal immunisation.
Pigs were immunised intraperitoneally with ovalbumin in Freund's complete adjuvant and subsequently challenged intratracheally with ovalbumin. The results show that the group receiving twoExpand
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