L. E. Drabkina

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The transfecting activity of linear λ DNA is 100 times higher in calcium treated E. coli K 12 (λi434) than in non-lysogenic strains: the levels of transfection are 1–2.107 and 1–2.105 infective centers per 1 μg of λ DNA, respectively. The high efficiency of lysogenic cells transfection is not due to the spontaneously liberated “helper” phage. Evidently, it(More)
Transfection efficiency of a number of lambda DNA samples differing in ring to linear molecules ratio was determined. Graphic extrapolation to the zero content of linear molecules showed that efficiency of ring molecules did not exceed 5% of that of linear molecules. Probably, this difference is caused by more fast penetration of linear molecules into the(More)
Infectivity of linear lambdaDNA molecules is proved to be about a hundred times higher in calcinated E. coli K12 (lambai434) than in E. coli K12(lambda-): the levels of transfection were 1-3-10(7) and 1-2-10(5) infective centers per 1 mug DNA, respectively. In E. coli JC 5743 rec B21 defective for exonucleases I and V the level of transfection was(More)
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