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Comparative 16S rRNA analysis of lake bacterioplankton reveals globally distributed phylogenetic clusters including an abundant group of actinobacteria.

• Frank Oliver Glöckner, E. F. Zaichikov, +4 authors Robert Amann
• Applied and environmental microbiology
• 2000

In a search for cosmopolitan phylogenetic clusters of freshwater bacteria, we recovered a total of 190 full and partial 16S ribosomal DNA (rDNA) sequences from three different lakes (Lake… (More)

Mapping of catalytic residues in the RNA polymerase active center.

• Eugeny Zaychikov, Eugene D. Martin, +7 authors Arkady Mustaev
• Science
• 1996

When the Mg2+ ion in the catalytic center of Escherichia coli RNA polymerase (RNAP) is replaced with Fe2+, hydroxyl radicals are generated. In the promoter complex, such radicals cleave template DNA… (More)

Influence of Mg2+ and temperature on formation of the transcription bubble.

• Evgeny Zaychikov, L Denissova, Thomas Meier, Maik Goette, Hermann Heumann
• The Journal of biological chemistry
• 1997

The transcription bubble formed in the binding complex of T7A1 promoter upon Escherichia coli RNA polymerase was analyzed by chemical probes, namely by single-strand specific reagents, to map the… (More)

Translocation of the Escherichia coli transcription complex observed in the registers 11 to 20: "jumping" of RNA polymerase and asymmetric expansion and contraction of the "transcription bubble".

• Evgeny Zaychikov, L Denissova, Hermann Heumann
• Proceedings of the National Academy of Sciences…
• 1995

Translocation of DNA-dependent RNA polymerase along the DNA template during RNA synthesis encompasses continuous as well as discontinuous steps. This is demonstrated by chemical probing of… (More)

Modular organization of the catalytic center of RNA polymerase.

• Arkadv Mustaev, Maxim Kozlov, Vadim Markovtsov, Eugeny Zaychikov, L Denissova, A. D. Goldfarb
• Proceedings of the National Academy of Sciences…
• 1997

The Fe2+ ion that specifically replaces Mg2+ in the active center of RNA polymerase generates reactive hydroxyl radicals that cause highly localized cleavage of polypeptide chains. Mapping of the… (More)

Hydroxyl radical footprinting.

• Evgeny Zaychikov, P Schickor, L Denissova, Hermann Heumann
• Methods in molecular biology
• 2001

Escherichia coli RNA polymerase translocation is accompanied by periodic bending of the DNA.

• Evgeny Zaychikov, L Denissova, Reinhard Guckenberger, Hermann Heumann
• Nucleic acids research
• 1999

RNA polymerase was halted in consecutive registers of RNA synthesis ranging from registers 11 to 68. Non-denaturing gel electrophoresis shows that the mobility of the complexes varies (up to 15%),… (More)

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