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A simple, inexpensive heating circuit is described for use with a warmed microscope stage, small tissue bath, and heated tissue culture chamber. A major consideration in the design of the apparatus is a compatability with electrophysiological studies. Thus, proper shielding and low profile for microelectrode positioning are featured.
Cultures established from mechanically dissociated neonatal mouse brains were found to be suitable for electrophysiological investigation of drug action. During culture most cells were aggregated into either monolayer regions or thick cords joining monolayer regions. A few cells remained isolated. The neurones in the monolayer regions were distinguished… (More)