L Bonamusa

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1. Fructose 2,6-bisphosphate (fru-2,6-P2) has been measured in liver and muscle of gilthead sea bream fish, Sparus aurata. 2. The fru-2,6-P2 levels in liver depend on the diet given to the fish: in fish fed a high carbohydrate diet, the fru-2,6-P2 levels are higher than any one previously reported. These changes are associated with differences in the(More)
1. Glycogen phosphorylase purified from muscle of mullet (Liza ramada) has been kinetically characterized. 2. Kinetic analysis for the substrates glucose-1-P and glycogen showed no homotropic co-operativity. AMP exhibited only a slight homotropic co-operative behaviour, although it caused a decrease in the Km for glucose-1-P. 3. Glucose, ATP and glucose-6-P(More)
1. Skeletal muscle glycogen phosphorylase b has been purified from Liza ramada (mullet). 2. The Mr of the purified enzyme subunit was found to be 97,000. By gel filtration a relative Mr of 190,000 was found. 3. Proteolytic digestion of 32P-phosphorylated mullet phosphorylase gave a [32P]-labelled peptide which is observed to contain Ser, its sequence being(More)
OBJECTIVE To evaluate the analytical reliability and accuracy as well as the practicability of the Glucocard Memory 2 glucose meter, intended to the control of the diabetic patient. DESIGN Descriptive, crossover study. To validate an analytical instrument according to guidelines of the Spanish Society of Clinical Biochemistry and Molecular Pathology. (More)
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