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Identification of structurally diverse methanofuran coenzymes in methanococcales that are both N-formylated and N-acetylated.
This work uses liquid chromatography coupled with high-resolution mass spectrometry and a variety of biochemical methods to deduce the unique structures of MFs present in four different methanogen in the order Methanococcales, the first detailed characterization of the MF occurring in methanogens of this order.
A Pterin-Dependent Signaling Pathway Regulates a Dual-Function Diguanylate Cyclase-Phosphodiesterase Controlling Surface Attachment in Agrobacterium tumefaciens
This study provides the first prokaryotic example of a pterin-mediated signaling pathway and a new model for the regulation of dual-function DGC-PDE proteins in the model pathogen Agrobacterium tumefaciens.
In vitro phosphinate methylation by PhpK from Kitasatospora phosalacinea.
- W. J. Werner, Kylie D. Allen, K. Hu, G. Helms, Brian S Chen, Susan C Wang
- Chemistry, BiologyBiochemistry
- 25 October 2011
The activity of the P-methyltransferase enzyme, PhpK, is demonstrated from the phosalacine producer Kitasatosporaphosalacinea to give rise to the only carbon-phosphorus-carbon linkage known to occur in nature.
Initial characterization of Fom3 from Streptomyces wedmorensis: The methyltransferase in fosfomycin biosynthesis.
Discovery of Multiple Modified F430 Coenzymes in Methanogens and Anaerobic Methanotrophic Archaea Suggests Possible New Roles for F430 in Nature
- Kylie D. Allen, G. Wegener, Robert H. White
- BiologyApplied and Environmental Microbiology
- 8 August 2014
Liquid chromatography–high-resolution mass spectrometry is used to identify several new structural variants of F430 in the cell extracts of selected methanogens and ANME, indicating that F430-related molecules have additional functions in nature and will inspire further research to determine the biochemical role(s) of these variants and the pathways involved in their biosynthesis.
Biogenic formation of amorphous carbon by anaerobic methanotrophs and select methanogens
Homocysteine is biosynthesized from aspartate semialdehyde and hydrogen sulfide in methanogenic archaea.
- Kylie D. Allen, Danielle V Miller, Benjamin J. Rauch, J. Perona, Robert H. White
- 11 May 2015
It is demonstrated that Methanocaldococcus jannaschii can biosynthesize homocysteine from aspartate semialdehyde and hydrogen sulfide and confirmed the genes involved in this new pathway in Methanosarcina acetivorans.
Spectroscopic characterization and mechanistic investigation of P-methyl transfer by a radical SAM enzyme from the marine bacterium Shewanella denitrificans OS217.
Identification of a Unique Radical S-Adenosylmethionine Methylase Likely Involved in Methanopterin Biosynthesis in Methanocaldococcus jannaschii
It is proposed that this archaeal radical SAM methylase employs a previously uncharacterized mechanism for methylation, using methylenetetrahydrofolate as a methyl group donor.
Investigation of enzymatic C–P bond formation using multiple quantum HCP nuclear magnetic resonance spectroscopy
- K. Hu, W. J. Werner, Kylie D. Allen, Susan C Wang
- ChemistryMagnetic resonance in chemistry : MRC
- 1 April 2015
A combination of stable isotopes and two‐dimensional nuclear magnetic resonance spectroscopy is utilized to obtain further insight into the catalytic mechanism of PhpK, the P‐methyltransferase responsible for the formation of the second C–P bond in l‐phosphinothricin.