Kyeong-Jun Lee

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The premise that a dietary dipeptide approach will improve the understanding of amino acid utilization in the fastest-growing vertebrate, the teleost fish, was tested by examining the muscle free amino acid (FAA) pool and enzyme activities, in concert with growth response, when dietary amino acids were provided in free, dipeptide or protein molecular forms.(More)
In vitro studies of brush-border intestinal transport of dipeptides and cytoplasmic hydrolysis in fish suggest that these processes could be key mechanisms in the absorption and utilization of nutrients for growth. However, in vivo experimentation to study the nutritional importance of these processes was needed. We compared three dietary formulations based(More)
We have conducted studies with juvenile yellow perch (Perca flavescens) over a period of 20 weeks to address the question of the interaction between water- and lipid-soluble antioxidant vitamins. Fish (2.25+/-0.14 g) were divided into twelve groups, and triplicate groups were fed one of four casein-based, semi-purified diets formulated to contain low or(More)
a University of Idaho, Aquaculture Research Institute, Hagerman Fish Culture Experiment Station, 3059-F National Fish Hatchery Road, Hagerman, Idaho 83332, USA b Jeju National University, Department of Marine Life Science, Jeju 690-756 and Marine and Environmental Research Institute, Jeju 695-814, South Korea c United States Department of Agriculture,(More)
There is a need to measure dietary transfer of gossypol and its metabolite, gossypolone in aquatic animals because of common use of cottonseed meal as a feed ingredient and fertilizer. The analytical method for gossypol and gossypolone enantiomers, therefore, becomes important. HPLC techniques have been developed by using mainly UV detection. We(More)
Five experimental diets containing increasing proportions of cottonseedmeal (CSM) protein (0, 25, 50, 75 and 100%; diets 1 to 5, respectively) toreplace fish meal (FM) protein were formulated for intensive culture of tilapiaOreochromis sp. Each diet was fed to three replicategroupsof fish (mean weight ± SE = 11.3 ± 3.9 g) in30L aquaria connected as a closed(More)
Golden shiners (Notemigonus crysoleucas) require a dietary source of ascorbic acid (AA) for growth or survival, depending on diet composition. However, no quantitative requirements of golden shiners for AA for growth, health or survival have been determined, and specific deficiency signs have not been observed. The purpose of this study was to determine the(More)
Apparent digestibility coefficients (ADCs) of dry matter, crude protein, crude lipid, nitrogen-free extract, and energy in selected carbohydrate sources including wheat flour (WF), α-potato starch (PS), α-corn starch (CS), Na alginate (AL), dextrin (DEX), and carboxymethyl cellulose (CMC) were determined for olive flounder. The olive flounder averaging 150(More)
Gossypol is an antifertilizing agent in males and females. However, gossypol and its metabolite, gossypolone, have also gained interest because of their anticarcinogenic activities. This paper examines for the first time both enantiomers of tissue gossypol and gossypolone in mature rainbow trout fed two diets containing low (15%) and high (60%) levels of(More)
In vitro studies of brush-border intestinal transport of dipeptides and cytoplasmic hydrolysis in fish suggest that these processes could be key mechanisms in the absorption and utilization of nutrients for growth. However, in vivo experimentation to study the nutritional importance of these processes was needed. We compared three dietary formulations based(More)