Kumiko Miyajima

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A flexible biosensor using a phospholipid polymer to immobilization of glucose oxidase (GOD) was fabricated and tested. At first, an enzyme membrane formed by immobilizing GOD onto a porous polytetrafluoroethylene (PTFE) membrane using the phospholipid polymer (2-methacryloyloxyethyl phosphorylcholine (MPC) copolymerized with 2-ethylhexylmethacrylate(More)
A soft contact-lens (SCL) biosensor for in-situ tear sugar monitoring was fabricated and tested. In contrast to the previously reported continuous glucose monitoring (CGM) devices, we focused on the relationship between blood glucose and tear glucose. The SCL biosensor was constructed by applying microelectromechanical systems (MEMS) techniques to(More)
A 2-dimensional imaging system was constructed and applied in measurements of gaseous ethanol emissions from the human palm. This imaging system measures gaseous ethanol concentrations as intensities of chemiluminescence by luminol reaction induced by alcohol oxidase and luminol-hydrogen peroxide-horseradish peroxidase system. Conversions of ethanol(More)
A tonometric biosensor for glucose was constructed using a chemo-mechanical reaction unit and a differential pressure sensor. The reaction unit was fabricated by using both liquid and gas cells separated by an enzyme diaphragm membrane, in which glucose oxidase was immobilized onto the single (gas cell) side of the dialysis membrane. By applying glucose(More)
A contact lens (CL) biosensor for in situ monitoring of tear glucose was fabricated and tested. Biocompatible 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer and polydimethyl siloxane (PDMS) were employed as the biosensor material. The biosensor consists of a flexible Pt working electrode and a Ag/AgCl reference/counter electrode, which were formed(More)
A simple application of chemiluminescence (CL) to multicomponent analysis of glucose, glutamic acid and lactic acid for food analysis using flow injection analysis (FIA) is described. The oxidative enzyme for each analyte and horseradish peroxidase (HRP) was employed. The sample solutions were analysed by the catalytic reaction on(More)
1Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8510, Japan 2Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai Chiyoda-ku, Tokyo 101-0062, Japan 3School of Science and Technology, Meiji University, 1-1-1 Higashi-Mita, Tama-Ku,(More)
An optical fiver gas-phase biosensor (bio-sniffer) for assessment of indoor formaldehyde was fabricated and tested. The bio-sniffer measures formaldehyde vapor as fluorescence of reduced nicotinamide adenine dinucleotide (NADH), which is the product of formaldehyde dehydrogenase (FALDH) reaction. Usually, an enzyme loses its specific activity in the gas(More)
A fiber optic bio-sniffer (biochemical gas sensor) for alcohol gas monitoring with high sensitivity and high selectivity was fabricated and tested. The bio-sniffer is a gas sensor that uses molecular recognition of enzyme to improve selectivity. Usually, enzyme loses activity in the gas phase. Applying a flow-cell with a gas-intake window to the sensing(More)
Dynamic fluoroimmunoassay with a flow-through system using optical fiber probes consisting of polystyrene was developed and applied to a quantitative detection of E. coli O157:H7. The system measures E. coli as fluorescence of sandwich-type immune complexes formed by capture antibodies immobilized on the surface of the probe, E. coli cells, and(More)