Kumar Patra

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Currently used protocols for the extraction of filarial parasite DNA from mosquito samples are tedious and involve extensive use of expensive and hazardous chemicals. Therefore, in order to arrive at a simple procedure, four different methods (A, B, C and D) were tried for the extraction of DNA from mosquitoes infected with filarial parasite, Brugia malayi.(More)
In India, Mass Drug Administration is on going towards elimination of lymphatic filariasis in many areas, which might lead to intense selection pressure on the parasite populations and their genetic restructuring. This calls for molecular finger printing of Wuchereria bancrofti parasite populations at national level and monitoring genetic changes in the(More)
Biting and feeding behavior of malaria vectors were studied in nine villages (5 from Jeypore zone and 4 from Malkangiri zone) of Koraput District. Man biting catches comprised of 16 anopheline species including the incriminated vectors of this area: An. fluviatilis, An. annularis and An. culicifacies. An. fluviatilis was predominant and biting of this(More)
A village scale trial was carried out to evaluate the efficacy of bed-nets impregnated with lambdacyhalothrin, at the dose of 0.025 g/m2, in reducing malaria transmission in villages of Koraput District of Orissa, India, inhabited by tribals. The nets were distributed before peak transmission season. There was an overall decline in the parasite rate in all(More)
The role of different anophelines in malaria transmission in two geoclimatic zones, viz., Jeypore and Malkangiri plateau of Koraput distt., which are endemic for malaria, was studied. Out of 10,110 specimens belonging to 9 species collected and dissected over a period of 12 months by different methods in nine study villages, natural infection (sporozoites(More)
Human kinetic energy can be transferred in a number of ways. Human energy is most commonly used to propel bicycles, but can also be used to generate electricity and power hand-crank tools. The articles on this page are about human kinetic energy and its many uses. Gravitational force acts on everybody that is present on earth, whether it's stationary or(More)
BACKGROUND & OBJECTIVES Periodic monitoring of vector population for infection and infectivity rates is central to the evaluation of the filariasis elimination strategies in endemic areas to monitor the success of MDA and also to establish endpoints for intervention. The main objective of this study was to develop a RT-PCR assay, based on L3 stage-specific(More)
The susceptibility status of malaria vectors to HCH and DDT was studied in Malkangiri district which has been highly endemic for malaria since many decades. An. fluviatilis is the major malaria vector and An. culicifacies is a secondary vector in the area. HCH is being used in malaria control programme from 1972 onwards. Before to that, DDT was used. This(More)
An Hha 1 based polymerase chain reaction (PCR) assay developed for the detection of Brugia malayi, the causative agent of Brugian lymphatic filariasis, was evaluated for its sensitivity in the laboratory and for its usefulness in measuring changes in transmission of the disease in the field. Laboratory studies showed that the new assay was highly sensitive(More)
In order to identify novel conserved integral membrane and other membrane-associated proteins of Plasmodium falciparum, lambda gt11-P. falciparum DNA library phages were immunoscreened with convalescent-phase mouse sera and rabbit antiserum against Triton X-114-soluble proteins of P. falciparum. One recombinant phage clone, L857, reacted with both of the(More)