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Electrospinning techniques enable the production of continuous fibers with dimensions on the scale of nanometers from a wide range of natural and synthetic polymers. The number of recent studies regarding electrospun polysaccharides and their derivatives, which are potentially useful for regenerative medicine, is increasing dramatically. However,(More)
Myocardial infarction causes a high rate of morbidity and mortality worldwide, and heat shock proteins as molecular chaperones have been attractive targets for protecting cardiomyoblasts under environmental stimuli. In this study, in order to enhance the penetration of heat shock protein 27 (HSP27) across cell membranes, we fused HSP27 with transcriptional(More)
Nonwoven matrices of silk fibroin (SF) nanofibers were prepared by electrospinning a regenerated SF solution, followed by treatment with solvent vapor including water, methanol, ethanol, and propanol. Structural changes of solvent vapor-treated SF nanofibers were investigated in a time-resolved manner using IR spectroscopy. Conformational transitions of SF(More)
Alginate is a biomaterial that has found numerous applications in biomedical science and engineering due to its favorable properties, including biocompatibility and ease of gelation. Alginate hydrogels have been particularly attractive in wound healing, drug delivery, and tissue engineering applications to date, as these gels retain structural similarity to(More)
Therapeutic angiogenesis is a promising approach to treat patients with cardiovascular disease, and will likely be critical to engineering large tissues. Many growth factors have been found to play significant roles in angiogenesis, and vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) are the most extensively investigated(More)
The intracellular delivery of small interfering RNA (siRNA) plays a key role in RNA interference (RNAi) and provides an emerging technique to treat various diseases, including infectious diseases. Chitosan has frequently been used in gene delivery applications, including siRNA delivery. However, studies regarding the modification of chitosan with antibodies(More)
Nano-sized vesicular systems (nanoparticles), ranging from 10 nm to 1000 nm in size, have potential applications as drug delivery systems. Successful clinical applications require the efficient intracellular delivery of drug-loaded nanoparticles. Here we describe N-acetyl histidine-conjugated glycol chitosan (NAcHis-GC) self-assembled nanoparticles as a(More)
PURPOSE We hypothesize that the controlled delivery of rhVEGF using a microsphere/hydrogel combination system could be useful to achieve active blood vessel formation in the ischemic hindlimb mouse model, which is clinically relevant for therapeutic angiogenesis without multiple administrations. METHODS A combination of poly(d,l-lactide-co-glycolide)(More)
It has been challenging to prepare polymeric microspheres with controlled porous structures for many biomedical applications, particularly for pulmonary drug delivery. Here, we report the use of bovine serum albumin (BSA) as an osmotic agent in order to control the porous structure of poly(D,L-lactide-co-glycolide) (PLGA) microspheres prepared by a double(More)
It was hypothesized that nanoscale adhesion ligand spacing regulates cell adhesion, proliferation, and differentiation, and that this control can be decoupled from the overall ligand density. Alginate was chemically modified with a peptide containing the cell adhesion sequence arginine-glycine-aspartic acid (RGD), and the nanoscale spacing of RGD ligands in(More)