Krzysztof Kazimierczuk

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A strategy for complete backbone and side-chain resonance assignment of disordered proteins with highly repetitive sequence is presented. The protocol is based on three resolution-enhanced NMR experiments: 5D HN(CA)CONH provides sequential connectivity, 5D HabCabCONH is utilized to identify amino acid types, and 5D HC(CC-TOCSY)CONH is used to assign the(More)
Theory Figure S1. (a) The true echo time signals from Eq. 2c and (b) the corresponding spectrum (Eq. 3c). Small ϕ=0.15π is used to illustrate the effect of non-zero phase on the signal in the time and frequency domains. The virtual-echo time-domain presentation given by Eq. 1 should be distinguished from the traditional true-echo signal previously suggested(More)
Pulsed gradient spin echo (PGSE) is a well-known NMR technique for determining diffusion coefficients. Various signal processing techniques have been introduced to solve the task, which is especially challenging when the decay is multiexponential with an unknown number of components. Here, we introduce a new method for the processing of such types of(More)
Multidimensional NMR spectroscopy requires time-consuming sampling of indirect dimensions and so is usually used to study stable samples. However, dynamically changing compounds or their mixtures commonly occur in problems of natural science. Monitoring them requires the use multidimensional NMR in a time-resolved manner - in other words, a series of quick(More)
Resonance assignment is a prerequisite for almost any NMR-based study of proteins. It can be very challenging in some cases, however, due to the nature of the protein under investigation. This is the case with intrinsically disordered proteins, for example, whose NMR spectra suffer from low chemical shifts dispersion and generally low resolution. For these(More)
A group of signal reconstruction methods, referred to as compressed sensing (CS), has recently found a variety of applications in numerous branches of science and technology. However, the condition of the applicability of standard CS algorithms (e.g., orthogonal matching pursuit, OMP), i.e., the existence of the strictly sparse representation of a signal,(More)
Rapid development of sparse sampling methodology offers dramatic increase in power and efficiency of magnetic resonance techniques in medicine, chemistry, molecular structural biology, and other fields. We suggest to use available yet usually unexploited prior knowledge about the phase and the causality of the sparsely detected NMR signal as a general(More)
Binuclear polypyridine ruthenium compounds have been shown to slowly intercalate into DNA, following a fast initial binding on the DNA surface. For these compounds, intercalation requires threading of a bulky substituent, containing one Ru(II), through the DNA base-pair stack, and the accompanying DNA duplex distortions are much more severe than with(More)
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