Krzysztof Fiedoruk

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AIMS To assess the prevalence and properties of Bacillus thuringiensis isolated from the intestines of small mammals. METHODS AND RESULTS Bacillus thuringiensis was found in 11% of rodents and 17% of insectivores. Using PFGE of chromosomal DNA, SDS-PAGE of whole-cell proteins and biochemical tests (API system), 12 isolates and three reference strains were(More)
PURPOSE Determination of bacterial composition in the oral cavity of patients with removable dentures and with own dentition (without dentures). MATERIAL AND METHODS Bacteriological investigations were performed in 55 patients from the department of internal medicine (32 diabetic patients) and 40 patients treated in surgical department (25 patients with(More)
Type II toxin-antitoxin systems (TAs) are bicistronic operons ubiquitous in prokaryotic genomes, displaying multilevel association with cell physiology. Various possible functions have been assigned to TAs, ranging from beneficial for their hosts, such as a stress response, dormancy and protection against genomic parasites, to detrimental or useless(More)
PURPOSE The aim was to determine the fungi occurrence rate in the oral cavity of denture wearer patients in comparison to those without dentures. MATERIAL AND METHODS The examinations were conducted in patients treated in two clinical departments of the University Hospital. Demographic data and those connected with basic diseases were collected and the(More)
The genome sequence of the entomopathogen Bacillus thuringiensis subsp. thuringiensis strain IS5056 was determined. The chromosome is composed of 5,491,935 bp. In addition, IS5056 harbors 14 plasmids ranging from 6,880 to 328,151 bp, four of which contain nine insecticidal protein genes, cry1Aa3, cry1Ab21, cry1Ba1, cry1Ia14, cry2Aa9, cry2Ab1, vip1, vip2,(More)
Escherichia albertii has been recently recognized as an emerging human and bird enteric pathogen. Here, we report the first complete chromosome nucleotide sequence of a clinical isolate of E. albertii strain KF1, which may provide information about the pathogenic potential of this new species and the mechanisms of evolution of enteropathogenic Escherichia(More)
Entomopathogenic Bacillus thuringiensis is closely related to Bacillus cereus, a human pathogen known to cause emesis and diarrhea. Standard detection methods do not distinguish these bacilli. Hemolysin BL (hbl) and non-hemolytic enterotoxin (nhe) genes that encode, respectively, HBL and NHE enterotoxins, are known to be harbored in both bacterial species,(More)
OBJECTIVES The purpose of this study was to determine the main causative agents of community-acquired acute diarrhoea in children using conventional methods and PCR. METHODS Stool samples were collected from 100 children under 5 years of age with acute diarrhoea during the autumn-winter period of 2010-2011. Rotaviruses and adenoviruses were detected by(More)
Although melanin is known for protecting living organisms from harmful physical and chemical factors, its synthesis is rarely observed among endospore-forming Bacillus cereus sensu lato. Here, for the first time, we reported that psychrotolerant Bacillus weihenstephanensis from Northeastern Poland can produce melanin-like pigment. We assessed(More)
The number of foodborne intoxications caused by emetic Bacillus cereus isolates has increased significantly. As such, rapid and reliable methods to identify emetic strains appear to be clinically relevant. In this study, intact cell matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was used to differentiate emetic(More)