Kristi L Budzinski

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The size of a synaptic vesicle (SV) is generally thought to be determined by the amount of lipid and membrane protein it contains. Once formed, it is thought to remain constant in size. Using fluorescence correlation spectroscopy and cryogenic electron microscopy, we show that glutamatergic vesicles reversibly increase their size upon filling with(More)
Uptake of neurotransmitters into synaptic vesicles is driven by the proton gradient established across the vesicle membrane. The acidification of synaptic vesicles, therefore, is a crucial component of vesicle function. Here we present measurements of acidification rate constants from isolated, single synaptic vesicles. Vesicles were purified from mice(More)
Synaptosomes are intact, isolated nerve terminals that contain the necessary machinery to recycle synaptic vesicles via endocytosis and exocytosis upon stimulation. Here we use this property of synaptosomes to load quantum dots into synaptic vesicles. Vesicles are then isolated from the synaptosomes, providing a method to probe isolated, individual synaptic(More)
This Communication reports real-time sizing of nanoparticles in microfluidic systems using confocal correlation spectroscopy (CCS). CCS can be used to measure the size of both fluorescent and nonfluorescent particles at low concentrations (<nanomolar) and in small volumes (<femtoliters). With advances in nonfluorescent modes of single-particle and(More)
VOLUME 34 NUMBER 3 MARCH 2016 NATURE BIOTECHNOLOGY To the Editor: Awareness and understanding about environmental risks have grown considerably in recent years, resulting in public policies and private sector efforts to encourage further development of environmentally sustainable business practices. For companies, the business benefits of environmentally(More)
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