Kristen Trett

Learn More
MOTIVATION The arbor morphologies of brain microglia are important indicators of cell activation. This article fills the need for accurate, robust, adaptive and scalable methods for reconstructing 3-D microglial arbors and quantitatively mapping microglia activation states over extended brain tissue regions. RESULTS Thick rat brain sections (100-300 µm)(More)
Microglia are Central Nervous System (CNS) cells that are similar to macrophages. They undergo characteristic changes in cell arbor morphology in response to tissue perturbation. Ensembles of microglia exhibit a progression of arbor morphologies. Our goal is to discover these progressions and determine the underlying arbor features from 3-D multi-channel(More)
BACKGROUND There is a need for effective computational methods for quantifying the three-dimensional (3-D) spatial distribution, cellular arbor morphologies, and the morphological diversity of brain astrocytes to support quantitative studies of astrocytes in health, injury, and disease. NEW METHOD Confocal fluorescence microscopy of multiplex-labeled(More)
The morphologies of microglial arbors in brain tissue are important indicators of their state of activation, and their spatial distribution indicates patterns of tissue perturbation. This paper presents a non-parametric Bayesian approach to unsupervised quantitative profiling of microglial activation states, and mapping their 3-D spatial distributions(More)
  • 1