Kristen Kaiser

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Twenty-one enzymes of different metabolic systems were measured in the rabbit fast-twitch tibialis anterior (TA) muscle after electrical stimulation (10 Hz, 24 h/day) for 1 day to 10 wk. Nine analytical methods are either new, (3-oxoacid CoA-transferase, branched-chain-amino-acid aminotransferase, carnitine acetyltransferase, thiolase), improved (glutamate(More)
Individual muscle fibers from the biceps of five individuals were analyzed for 10 different enzymes. For each person, lactic dehydrogenase and adenylokinase were first measured in 50 to 100 fibers. From each large group, 20 or more fibers, representing the full range of enzyme activity, were analyzed in duplicate for the other eight enzymes (glycogen(More)
The effects of thyrotoxicosis on the contractile properties of soleus muscle were examined in rats given 3 mg of T4 and 1 mg of T3 per kg of diet for 6--8 wk. Thyrotoxicosis induced significant decreases in isometric twitch contraction time (CT), one-half relaxation time, and peak twitch tension. The Ca2+ uptake activity of the sarcoplasmic reticulum (SR)(More)
Rat plantaris and soleus muscles were stimulated intensely in vivo for 1 and 15 min, freeze-clamped, and freeze-dried, and individual fibers were dissected free. Fibers, assigned to four groups on the basis of lactate dehydrogenase and malate dehydrogenase, were each separately analyzed for ATP, P-creatine, glycogen, glucose, glucose-6-phosphate(More)
Individual muscle fibers from the rat anterior tibialis and soleus muscles were each analyzed in duplicate for lactate dehydrogenase (LDH, EC, malate dehydrogenase (MDH, EC, 3-hydroxyacyl-CoA dehydrogenase (EC, fumarate hydrotase (EC, glycogen phosphorylase (EC, 6-phosphofructokinase (EC, pyruvate(More)