Kristen K. Briggs

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OBJECTIVE To determine the in vivo biocompatibility of septal neocartilage constructs developed in vitro by an alginate intermediate step. STUDY DESIGN Prospective, animal model. SETTING Research laboratory. SUBJECTS AND METHODS A murine model was used to examine the maturation of neocartilage constructs in vivo. Chondrocytes collected from patients(More)
OBJECTIVES/HYPOTHESIS Tissue-engineered septal cartilage may provide a source of autologous cartilage for repair of nasal defects. Production of clinically useful neocartilage involves multiple steps that include manipulating the culture environment. Partial pressure of oxygen (ppO(2) ) is a property that has been shown to influence cartilage development.(More)
OBJECTIVES/HYPOTHESIS To localize quantitatively the major biochemical constituents of native adult human septal cartilage across whole septa. STUDY DESIGN Prospective, basic science. METHODS The nasal septa from seven cadavers were partitioned into 24 separate regions: six from caudal to cephalic and four from dorsal to ventral. Biochemical assays were(More)
OBJECTIVE To determine and compare the bending moduli of native and engineered human septal cartilage. STUDY DESIGN Prospective, basic science. SETTING Research laboratory. SUBJECTS AND METHODS Neocartilage constructs were fabricated from expanded human septal chondrocytes cultured in differentiation medium for 10 weeks. Constructs (n = 10) and native(More)
OBJECTIVES Evaluate safety of autogenous engineered septal neocartilage grafts.Compare properties of implanted grafts versus in vitro controls. STUDY DESIGN Prospective, basic science. SETTING Research laboratory. METHODS Constructs were fabricated from septal cartilage and serum harvested from adult rabbits and then cultured in vitro or implanted on(More)
IMPORTANCE Cartilaginous craniofacial defects range in size and autologous cartilaginous tissue is preferred for repair of these defects. Therefore, it is important to have the ability to produce large size cartilaginous constructs for repair of cartilaginous abnormalities. OBJECTIVES To produce autologous human septal neocartilage constructs(More)
OBJECTIVE To test engineered and native septal cartilage for resistance to deformation and remodeling under sustained bending loads and to determine the effect of bending loads on the biochemical properties of constructs. STUDY DESIGN Prospective, basic science. SETTING Laboratory. SUBJECTS AND METHODS Human septal chondrocytes from 6 donors were used(More)
Tissue-engineered nasal septal cartilage may provide a source of autologous tissue for repair of craniofacial defects. Although advances have been made in manipulating the chondrocyte culture environment for production of neocartilage, consensus on the best oxygen tension for in vitro growth of tissue-engineered cartilage has not been reached. The objective(More)
OBJECTIVES (1) To show that extracellular matrix deposition in 3-dimensional culture of human septal chondrocytes cultured in a rotary bioreactor is comparable to the deposition achieved under static culture conditions. (2) To demonstrate that the biomechanical properties of human septal chondrocytes cultured in a bioreactor are enhanced with time and are(More)
OBJECTIVES Structural properties of tissue-engineered cartilage can be optimized by altering its collagen to sulfated glycosaminoglycan (sGAG) ratio with hyaluronidase. The objective was to determine if treatment of neocartilage constructs with hyaluronidase leads to increased collagen:sGAG ratios, as seen in native tissue, and improved tensile properties.(More)
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