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Soybean (Glycine soja var Beeson) formate dehydrogenase has been isolated, purified, and partially characterized by affinity chromatography. The enzyme is a dimer having a total molecular weight of 100,000 and a subunit weight of 47,000. It has activity over a broad pH range, is stable for months at 4 degrees C, and has K(m) values of 0.6 millimolar and 5.7(More)
Less than 3 per cent of the [1-14C]glycolate administered, orally to fasted and nonfasted male Wistar rats was excreted in the feces in 48 hr. This finding indicates that the glycolate was readily absorbed from the digestive tract. An average of 3 per cent of the administered [1-14C]glycolate was also recovered in 48 hr as urinary [14C]oxalate from fasted(More)
Oxalate oxidase (EC 1.2.3.4) was purified from beet stems and immobilized on concanavalin A. The bound enzyme showed a high resistance of denaturation and increased the storage stability at 4 degrees C. The immobilized oxidase showed a broad optimum at pH 3.5-5, compared to the free enzyme with a sharp optimum at pH 4.5. There was a 3-fold increase in the(More)
RNA interference (RNAi) has become a powerful tool for genetic screening in Drosophila. At the Drosophila RNAi Screening Center (DRSC), we are using a library of over 21,000 double-stranded RNAs targeting known and predicted genes in Drosophila. This library is available for the use of visiting scientists wishing to perform full-genome RNAi screens. The(More)
BACKGROUND Short sleep duration has been associated with greater risks of obesity, hypertension, diabetes, and cardiovascular disease. Also, common genetic variants in the human Circadian Locomotor Output Cycles Kaput (CLOCK) show associations with ghrelin and total energy intake. OBJECTIVES We examined associations between habitual sleep duration, body(More)
BACKGROUND Gene variants within regulatory regions are thought to be major contributors of the variation of complex traits/diseases. Genome wide association studies (GWAS), have identified scores of genetic variants that appear to contribute to human disease risk. However, most of these variants do not appear to be functional. Thus, the significance of the(More)
The metabolic pathway by which L-[14C1]phenylalanine, L-[14C1]tyrosine, L-[14C1]tryptophan, and L-[14C1]ascorbic acid are converted to [14C]oxalate have been investigated in the male rate. Only [14C]oxalate was detected in the urine of rats injected with L-[14C1]ascorbic acid, but [14C]-labeled oxalate, glycolate, glyoxylate, glycolaldehyde, glycine, and(More)