Greenhouse experiments were conducted to investigate the importance of tomato seeds as a route of pospiviroid dissemination. More than 7000 seeds were collected from tomato plants mechanically… (More)
During the past decade, pospiviroid outbreaks in tomato and sweet pepper crops have been regularly reported worldwide. Although seed transmission was often suspected, transmission from asymptomatic… (More)
Over the years, real-time PCR outflanked endpoint PCR in phytopathogen diagnostics, mainly because of the increase in sensitivity and timesaving aspects of the technique. However, a time consuming… (More)
In Belgium pospiviroids are routinely detected in various hosts. The most frequently found pospiviroids are: Citrus exocortis viroid (CEVd), Chrysanthemum stunt viroid (CSVd), Potato spindle tuber… (More)
Four pospiviroid detection methods consisting of a pair of RT-PCR (ANSES 1-2), a pair of real time RT-PCR (Botermans 1-2) and two single RT-PCR methods (Luigi and Olivier) were evaluated for their… (More)
Phytophthora nicotianae is a common and destructive pathogen of numerous ornamental, agronomic and horticultural crops such as tomato, tobacco and citrus. Three monobranched C13 alcohol alkoxylate… (More)