Krešimir Krnjević

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The late phase of long-term potentiation (LTP) and memory (LTM) requires new gene expression, but the molecular mechanisms that underlie these processes are not fully understood. Phosphorylation of eIF2alpha inhibits general translation but selectively stimulates translation of ATF4, a repressor of CREB-mediated late-LTP (L-LTP) and LTM. We used a(More)
1. The muscarinic depolarizing action of ACh on cortical neurones is associated with an increase in membrane resistance (mean DeltaV/DeltaR = 3.16 mV/MOmega).2. ACh also promotes repetitive firing by slowing repolarization after spikes.3. The depolarizing effect has a mean reversal level of -86.7 mV (with mean resting potential -56 mV).4. It is concluded(More)
1. In 400-microns-thick slices from young adult Sprague-Dawley rats, CA1 pyramidal layer neurons were studied by the whole-cell recording technique. The patch pipettes were filled most often with (in mM) 140 potassium gluconate, 2 MgCl2, and 0.2 guanosine triphosphate (GTP): in many cases, 2 mM ATP and/or 1.1 mM EGTA and 0.1 mM Ca were added. The slices(More)
A major goal of biomedical research is the identification of molecular and cellular mechanisms that underlie memory storage. Here we report a previously unknown signaling pathway that is necessary for the conversion from short- to long-term memory. The mammalian target of rapamycin (mTOR) complex 2 (mTORC2), which contains the regulatory protein Rictor(More)
BACKGROUND Although ethanol addiction is believed to be mediated by the mesolimbic dopamine system, originating from the ventral tegmental area (VTA), how acute ethanol increases the activity of VTA dopaminergic (DA) neurons remains unclear. METHOD Patch-clamp recordings of spontaneous firings of DA and GABAergic neurons in the VTA in acute midbrain(More)
N-Methyl-D-aspartate (NMDA), applied by superfusion to hippocampal slices evokes comparable peak inward currents in CA3 pyramidal cells of adult and 0-10 day-old rats. However, NMDA current-voltage plots from immature neurones do not consistently show the region of negative slope conductance characteristic of adult. Therefore at a critical stage of(More)
1. During whole-cell recordings from CA1 neurons of rat brain slices with electrodes containing only KMeSO4 and Hepes, brief anoxia (2-3 min) consistently evoked a hyperpolarization (delta V approximately 14 mV) and reduction in input resistance (delta R approximately -20%). 2. As in previous intracellular recordings, Dantrolene sodium (10 microM)(More)
1. Effects of anoxia (2-4 min of 95% N2-5% CO2) on membrane currents of CA1 neurons were studied by single-electrode voltage clamp in hippocampal slices (from Sprague-Dawley rats) kept in an interface-type chamber at 33.5 degree. 2. When recording with KCl electrodes at a holding potential (VH) near-70 mV, anoxia evoked a slow outward current [0.18 +/- 0.06(More)
GABA-mediated postsynaptic currents (IPSCs) were recorded from dopaminergic (DA) neurons of the ventral tegmental area (VTA) of rats, in acute brain slices, and from enzymatically or mechanically dissociated neurons. In young rats (3-10 d of age), where GABA is excitatory, glycine (1-3 microm) and taurine (10-30 microm) increased the amplitude of evoked(More)