Krasimir Slantchev

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We have established a primary cell culture of the marine demosponge Ircinia muscarum. The culture was started from a cell suspension obtained by a combination of mechanical chemical means. Microbial contamination was controlled by the use of a pool of antibiotics. Optical density, rather than hemocytometer count, is suggested to monitor the cellular growth.(More)
Eighteen sterols were identified in Botryllus schlossen, stanols being the main sterols. The sterol composition is in agreement with our recent paper on taxonomic separation of tunicates into three groups. Again we found in invertebrates of the Black Sea sterols with a (22Z)-double bond. This confirms the presence of such sterols in nature. The composition(More)
Sterols, volatiles and lipids were isolated and identified from lipophylic extracts from two tunicates, Styela sp. and Phallusia sp., occurring in the Eastern Mediterranean. Seventeen sterols were identified. The sterol composition of the two organisms appeared to be similar except for the concentrations of 5alpha-stanols. Both tunicates were characterized(More)
Eighteen sterols were identified in Botryllus schlosseri, stanols being the main sterols. The sterol composition is in agreement with our recent paper on taxonomic separation of tunicates into three groups. Again we found in invertebrates of the Black Sea sterols with a (22Z)-double bond. This confirms the presence of such sterols in nature. The composition(More)
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