Kouta Tsuchiyama

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Department of Chemistry and Biology Engineering, Fukui National College of Technology, Geshi-cho, Sabae-shi, Fukui 916-8507, Japan, Fiber Amenity Course, Graduate School of Engineering, University of Fukui, 3-9-1 Bunkyo, Fukui-shi, Fukui 910-8507, Japan, Laboratory of Biomacromolecular Chemistry, Department of Applied Biochemistry, Division of Applied Life(More)
The gene encoding organophosphorus hydrolase (OPH) from Flavobacterium species was expressed on the cell surface of Saccharomyces cerevisiae MT8-1 using a glycosylphosphatidylinositol (GPI) anchor linked to the C-terminal region of OPH. Immunofluorescence microscopy confirmed the localization of OPH on the cell surface, and fluorescence intensity(More)
Organophosphorus hydrolase (OPH) hydrolyzes organophosphorus esters. We constructed the yeast-displayed OPH using Flo1p anchor system. In this system, the N-terminal region of the protein was fused to Flo1p and the fusion protein was displayed on the cell surface. Hydrolytic reactions with paraoxon were carried out during 24 h of incubation of(More)
Organophosphorus compounds (OPs) such as pesticides, fungicides, and herbicides are highly toxic but are nevertheless extensively used worldwide. To detect OPs, we constructed a yeast strain that co-displays organophosphorus hydrolase (OPH) and enhanced green fluorescent protein (EGFP) on the cell surface using a Flo1p anchor system. OP degradation releases(More)
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