Kosuke Nakayama

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Organophosphorus hydrolase (OPH; EC is known to be capable of hydrolyzing a variety of organophosphorus compounds, such as sarin and paraoxon. We have developed a nerve agent decontamination method using OPH. The gene that encodes OPH was cloned from the bacterial strain Sphingobium fuliginis ATCC 27551, and several OPH gene fusion plasmids were(More)
The hydrolytic activities of organophosphorus hydrolase (OPH) toward nerve agents were investigated by using mutant OPH enzymes constructed by site-directed mutagenesis of the gene. The amino acids at position 136, 254, and 257 are known to influence the enzyme activity. The nucleotide sequencing of the OPH gene cloned in our previous study revealed that(More)
Several classes of mutants derived from facultative methylotrophs, Microcyclus eburneus and Protaminobacter ruber, accumulate 0.5–3.8 mg/ml of O-methyl-L-homoserine (OMH) in a medium containing methanol as sole carbon source. The wild-type parent strains also accumulate OMH when the medium is supplemented with L-homoserine; the accumulation of OMH by the(More)
The plasmid encoding His-tagged organophosphorus hydrolase (OPH) cloned from Sphingobium fuliginis was modified to be transferred back to this bacterium. The replication function of S. amiense plasmid was inserted at downstream of OPH gene, and S. fuliginis was transformed with this plasmid. The transformant produced larger amount of active OPH with His-tag(More)
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