Konstantin Konrad

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Cy5, an activated carboxyl cyanine fluorophore, was characterized by capillary electrophoresis (CE) using a semiconductor laser at 652 nm to induce fluorescence. Hydrolysis of the activated Cy5 in the presence of ammonia results in the formation of a mono- and diamide and a dicarboxylic acid. A Cy5-labeled oligonucleotide M13 primer for DNA sequencing(More)
Ramp-up of precision assembly lines is a cost-intensive and experience-driven task. Most of the time the knowledge how to effectively and efficiently setup an assembly line is intrinsic and is therefore neither shared nor reused by production experts. Almost no machine data is recorded until the correct functionality of the line is achieved and human(More)
Intramolecular base pairing at the 3' end of single-stranded DNA molecules can cause increased electrophoretic mobility. In DNA sequencing separations this can result in compressions, where multiple oligomers differing in length by one base comigrate, which complicate sequence analysis. Using a novel approach to the application of capillary electrophoresis(More)
The Tabor and Richardson strategy for enzymatic chain termination sequencing of DNA using relative peak intensity has been adapted to high performance capillary gel electrophoresis with laser induced fluorescence detection. This approach to DNA sequencing involves the use of only a single fluor and results in significant reduction in the time required to(More)
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