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  • Kohsuke Uchimura, Masayuki Miyazaki, Yuichi Nogi, Tohru Kobayashi, Koki Horikoshi
  • Biology, Medicine
  • Marine Biotechnology
  • 2009 (First Publication: 1 October 2010)
  • Four alginate lyase genes were cloned and sequenced from the genomic DNAs of deep-sea bacteria, namely members of Vibrio and Agarivorans. Three of them were from Vibrio sp. JAM-A9m, which encodedExpand
  • Tohru Kobayashi, Kohsuke Uchimura, Osamu Koide, Shigeru Deguchi, Koki Horikoshi
  • Biology, Medicine
  • Bioscience, biotechnology, and biochemistry
  • 2012 (First Publication: 23 March 2012)
  • An alkaline κ-carrageenase, Cgk-K142, was found in the culture broth of a deep-sea bacterium, Pseudoalteromonas tetraodonis JAM-K142. A gene for the enzyme was cloned and expressed. PurifiedExpand
  • Hiroki Shibasaki, Kohsuke Uchimura, Takeshi Miura, Tohru Kobayashi, Ron Usami, Koki Horikoshi
  • Medicine, Biology
  • Applied Microbiology and Biotechnology
  • 2014 (First Publication: 16 April 2014)
  • A novel chitinase (LpChiA) was purified to homogeneity from a culture of Laceyella putida JAM FM3001. LpChiA hydrolyzed colloidal chitin optimally at a pH of 4 in an acetate buffer and temperature ofExpand
  • Tohru Kobayashi, Kohsuke Uchimura, Shigeru Deguchi, Koki Horikoshi
  • Medicine, Biology
  • Applied and Environmental Microbiology
  • 2012 (First Publication: 27 January 2012)
  • ABSTRACT An inulinase-producing Microbulbifer sp. strain, JAM-3301, was isolated from a deep-sea sediment. An inulin operon that contained three open reading frames was cloned and sequenced. Two ofExpand