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Thymus-derived lymphocytes protect mammalian hosts against virus-or cancer-related cellular alterations through immune surveillance, eliminating diseased cells. In this process, T cell receptors (TCRs) mediate both recognition and T cell activation via their dimeric ␣␤, CD3⑀␥, CD3⑀␦, and CD3␨␨ subunits using an unknown structural mechanism. Here,(More)
G protein-activated inwardly rectifying potassium channel (GIRK) plays crucial roles in regulating heart rate and neuronal excitability in eukaryotic cells. GIRK is activated by the direct binding of heterotrimeric G protein βγ subunits (Gβγ) upon stimulation of G protein-coupled receptors, such as M2 acetylcholine receptor. The binding of Gβγ to the(More)
Non-negative Matrix Factorization (NMF) is a traditional unsupervised machine learning technique for decomposing a matrix into a set of bases and coefficients under the non-negative constraint. NMF with sparse constraints is also known for extracting reasonable components from noisy data. However, NMF tends to give undesired results in the case of highly(More)
The human Mediator coactivator complex interacts with many transcriptional activators and facilitates recruitment of RNA polymerase II to promote target gene transcription. The MED25 subunit is a critical target of the potent herpes simplex 1 viral transcriptional activator VP16. Here we determine the solution structure of the MED25 VP16-binding domain(More)
—Non-negative Tensor Factorization (NTF) is a widely used technique for decomposing a non-negative value tensor into sparse and reasonably interpretable factors. However, NTF performs poorly when the tensor is extremely sparse, which is often the case with real-world data and higher-order tensors. In this paper, we propose Non-negative Multiple Tensor(More)
omega-Grammotoxin SIA (GrTx) is a 36 amino acid residue protein toxin from spider venom that inhibits P/Q and N-type voltage-gated Ca(2+) channels by modifying voltage-dependent gating. We determined the three-dimensional structure of GrTx using NMR spectroscopy. The toxin adopts an "inhibitor cystine knot" motif composed of two beta-strands (Leu19-Cys21(More)
The action mechanism of sapecin, an antibacterial peptide with membrane permeabilization activity, was investigated. The dose dependence of the membrane permeabilization caused by sapecin was sigmoidal, suggesting that sapecin oligomerization leads to the membrane permeabilization. Solution nuclear magnetic resonance analysis of the sapecin-phospholipid(More)
—The amount and variety of multimedia data such as images, movies and music available on over social networks are increasing rapidly. However, the ability to analyze and exploit these unorganized multimedia data remains inadequate, even with state-of-the-art media processing techniques. Our finding in this paper is that the emerging social curation service(More)
Gating of ion channels is strictly regulated by physiological conditions as well as intra/extracellular ligands. To understand the underlying structures mediating ion channel gating, we investigated the pH-dependent gating of the K(+) channel KcsA under near-physiological conditions, using solution-state NMR. In a series of (1)H(15)N-TROSY HSQC (transverse(More)
Phosphoinositide kinases regulate diverse cellular functions and are important targets for therapeutic development for diseases, such as diabetes and cancer. Preparation of the lipid substrate is crucial for the development of a robust and miniaturizable lipid kinase assay. Enzymatic assays for phosphoinositide kinases often use lipid substrates prepared(More)