Kobkul Laoteng

Learn More
BACKGROUND Up to now, there have been three published versions of a yeast genome-scale metabolic model: iFF708, iND750 and iLL672. All three models, however, lack a detailed description of lipid metabolism and thus are unable to be used as integrated scaffolds for gaining insights into lipid metabolism from multilevel omic measurement technologies (e.g.(More)
The genome-scale metabolic model <i>ilN800</i> of <i>Saccharomyces cerevisiae</i> and its validation: a scaffold to query lipid metabolism "The genome-scale metabolic model <i>ilN800</i> of <i>Saccharomyces cerevisiae</i> and its validation: a scaffold to query lipid metabolism". Notice: Changes introduced as a result of publishing processes such as(More)
Fatty acid elongation defective mutant was isolated from the ethyl methanesulfonate treated Hansenula polymorpha based on the growth ability. Using biochemical and genetic approaches, the mutant was characterized. When compared with the fatty acid phenotype of the parental strain, the differences in profile and content of fatty acids in V1 mutant were(More)
An integrative simultaneous saccharification and fermentation (SSF) modeling is a useful guiding tool for rapid process optimization to meet the techno-economic requirement of industrial-scale lignocellulosic ethanol production. In this work, we have developed the SSF model composing of a metabolic network of a Saccharomyces cerevisiae cell associated with(More)
Aspergillus oryzae is widely used for the industrial production of enzymes. In A. oryzae metabolism, transporters appear to play crucial roles in controlling the flux of molecules for energy generation, nutrients delivery, and waste elimination in the cell. While the A. oryzae genome sequence is available, transporter annotation remains limited and thus the(More)
Mucor circinelloides is an attractive oleaginous filamentous fungus because it can produce high content of triacylglycerol (TAG) rich in &#947;-linolenic acid (GLA). With the increasing attention in microbial oils, many experiments have been carried out in order to enhance storage lipid production in M. circinelloides. Availabilities of genome sequence,(More)
Using chemical mutagenesis, mutants of Hansenula polymorpha that were defective in fatty acid synthesis were selected based on their growth requirements on saturated fatty acid mixtures. One mutant (S7) was incapable of synthesizing polyunsaturated fatty acids (PUFA), linoleic and α-linolenic acids. A genetic analysis demonstrated that the S7 strain had a(More)
BACKGROUND The ∆(6)-desaturase gene, encoding a key enzyme in the biosynthesis of polyunsaturated fatty acids, has potential in pharmaceutical and nutraceutical applications. RESULTS The ∆(6)-desaturase gene has been isolated from a selected strain of Oomycetes, Pythium sp. BCC53698. The cloned gene (PyDes6) contained an open reading frame (ORF) of 1401(More)
  • 1