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Transmission of viruses via surface water is a major public health concern. To determine the viral concentration in rivers of a densely-populated area in Germany, the virus adsorption elution (VIRADEL) method was optimized for downstream PCR applications. Using a high-salt alkaline phosphate buffer for elution, the median recovery efficiency from spiked 1l(More)
BACKGROUND The clinical relevance of infections with the novel human coronavirus NL63 (HCoV-NL63) has not been investigated systematically. We therefore determined its association with disease in young children with lower respiratory tract infection (LRTI). METHODS AND FINDINGS Nine hundred forty-nine samples of nasopharyngeal secretions from children(More)
BACKGROUND The disease burden caused by recently identified respiratory viruses like HCoV-NL63 is unknown. OBJECTIVES We determined the burden of disease due to HCoV-NL63 infections using the population-based PRI.DE cohort of children under the age of 3 with lower respiratory tract infections (LRTIs). STUDY DESIGN In total 1756 respiratory samples, from(More)
Rabbits were immunized with a synthetic heptapeptide of the sequence Arg-Asn-Arg-Ser-Ser-Arg-Ser corresponding to the carboxy-terminal region of the SV40 viral proteins VP2 and VP3. The raised antibodies recognize the viral proteins in enzyme-linked immunosorbent (ELISA) and Western blot assay. Specificity of the antibodies were confirmed by competition(More)
One hundred and ninety-eight serial stool samples were collected from 27 infants and children hospitalized in Bochum, Germany with gastroenteritis due to serotype 1 rotavirus (RV). RV antigen and RV-specific antibodies (Ab) (IgA ELISA and RV Wa-neutralizing Ab) were measured. The prevalence of RV-Ab positive stool samples in RV patients did not differ from(More)
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