Klaus Spindler

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An epithelial cell line from Chironomus tentans exhibits acetylcholinesterase activity (specific activity 0.05–0.2 nkat/mg protein), which rises 30− to 40-fold after addition of 10−6 M 20-OH-ecdysone. The first visible increase occurs after 4 days of incubation with hormone. The enzyme has an apparent K m of 2.3±0.2×10−4 M for acetylthiocholine iodide as(More)
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