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Laser induced convection flows is a new and promising method to achieve better manipulation of mesoscale objects (above 1 mum and below 500 mum) in a liquid medium. The temperature gradient created by laser absorption generates natural and thermocapillary (or Marangoni) convection flows. These flows are used to perform the manipulation itself. In this(More)
Lentiviral transduction of oocytes or early embryos is an efficient strategy to generate transgenic rodents and livestock. We evaluated laser-based microdrilling (MD) of the zona pellucida, which is a physical barrier for viral infection, and subsequent incubation in virus suspension as a new route for lentiviral transgenesis in bovine. Lentiviral vectors(More)
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