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Seed-Specific Overexpression of an Endogenous Arabidopsis Phytoene Synthase Gene Results in Delayed Germination and Increased Levels of Carotenoids, Chlorophyll, and Abscisic Acid1
TLDR
To elucidate if the capacity to produce phytoene is limiting also in the seed of Arabidopsis, a gene coding for an endogenous phy toene synthase was cloned and coupled to a seed-specific promoter, and the effects of the overexpression were examined.
Interaction between composite elements in the napA promoter: both the B-box ABA-responsive complex and the RY/G complex are necessary for seed-specific expression
TLDR
The seed-specific activity of the napA promoter relies on the combinatorial interaction between the RY/G complex and the B-box ABA-responsive complex during the ABA response in seed development.
Disruption of an overlapping E-box/ABRE motif abolished high transcription of the napA storage-protein promoter in transgenic Brassica napus seeds
TLDR
Results imply that the E-box/ABRE-like sequence is a major motif of the napA promoter and suggest that the CAAACAC sequence is important for high activity of theNapin promoter.
Functional dissection of a napin gene promoter: identification of promoter elements required for embryo and endosperm-specific transcription
TLDR
The promoter region (−309 to +44) of the Brassica napus storage protein gene napA was studied in transgenic tobacco by successive 5′ as well as internal deletions fused to the reporter gene GUS and a cis element similar to the (CA)n element, found in other storage protein promoters, was identified.
Characterization of two Arabidopsis thaliana acyltransferases with preference for lysophosphatidylethanolamine
TLDR
This study represents the first description of plant acyltransferases with a preference for LPE, and it is suggested that the two AtLPEATs, with their different biochemical and expression properties, have different roles in membrane metabolism/homoeostasis.
Synthesis of ketocarotenoids in the seed of Arabidopsis thaliana.
TLDR
A cDNA coding for a gene necessary for synthesis of ketocarotenoids was cloned from the alga Haematococcus pluvialis and expressed in the seed of Arabidopsis thaliana, giving rise to seeds with a 4.6-fold relative increase of the total pigment and the three major keto-lutein were increased 13-fold compared to seeds of transgenic plants carrying only the beta-carotene-oxygenase construct.
Deletion analysis of a 2S seed storage protein promoter of Brassica napus in transgenic tobacco
TLDR
In tobacco, the napA promoter directed GUS activity early in the endosperm before any visible activity could be seen in the heart-shaped embryo, and during the transition from heart to torpedo stages, the main expression of GUS was localized to the embryo.
Distinct sequence elements in a napin promoter interact in vitro with DNA-binding proteins from Brassica napus
TLDR
Nuclear extracts obtained from developing seeds of oilseed rape were shown to contain several distinct DNA-binding proteins that were capable of interacting in vitro with oligonucleotide probes containing sequences related to motives in a napin gene promoter and its upstream region.
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