Kiyoshi Wako

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Maltohexaose producing amylase (EC 3.2.1.-) is the fourth known exo-amylase, the three previously known being glucoamylase, beta-amylase and Pseudomonas stutzeri maltotetraose producing amylase. The enzyme after release from Aerobacter aerogenes cells by 0.1% sodium lauryl sulfate extraction was purified by ammonium sulfate precipitation, DEAE-Sephadex(More)
To validate use of the common cotton-eared marmoset (Callithrix jacchus) in inhalation toxicity studies, its nasal morphology was examined. The nasal turbinates each consisted of one maxilloturbinate and one ethmoturbinate: these were more planar in structure than the comparable structures of rodents or dogs. The nasal cavity epithelia comprised squamous(More)
Since there is a possibility of inhaling the fibers of multi-wall carbon nanotube (MWCNT) without any agglomeration, it is important that the pulmonary toxicity is evaluated by intratracheal instillation without agglomeration. MWCNT suspended in an artificial lung surfactant (ALS) with or without grinding in an agate mortar was instilled once(More)
Air purifiers, which release positive and negative ions generated by an electric discharge into the air, have been widely used in common households. In this study, the developmental toxicity potential of the ionized air containing positive and negative ions was evaluated in SD rats [Crl:CD(SD)] following whole-body inhalation to obtain preliminary(More)
Air purifiers, which release positive and negative ions generated by electric discharge, are widely used in a variety of places. In this study, male and female SD rats [Crl:CD(SD)] were exposed by whole-body inhalation (6 hr/day) to ionized air containing positive and negative ions for at least 10 weeks before mating and throughout the mating, gestation,(More)
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